Streptococcus mutans and Streptococcus dentisani in dental biofilm of children with different caries status: a pilot study

Objective: Streptococcus mutans is one of the etiological agents associated with caries due to its ability tometabolize carbohydrates and resist acidic environments. On other hand, dentisani, showscharacteristics control inhibit growth cariogenic bacteria. Theaim this work was quantify levels dentisani from dentalbiofilm children related their situation. Material Methods: After identification morphologiccharacteristics reference strains performed, clinical isolates biofilm compatible these wereselected Polymerase Chain Reaction technique performed using species-specific primers. Biofilmsamples 25 21 without were collected S. andS. dentisani. Results: There statistically significant differences in groupand increased as severity carious lesion increased. By contrast, higher dentisaniwere found caries-free group, although no found. In addition,the decreased Conclusion: The increasein frequency group suggests possibility requiring minimum ofthis species dental show an actual protective effect. It must also be considered that effectmight intrinsic factors intraspecies genetic variability every individual. KEYWORDSBiofilm; Clinical isolates; Dental caries; dentisani; mutans.